Reduced glomerular apoptosis in FVL mice. (A-B) Frequency of apoptosis in glomeruli as determined by TUNEL assay. Apoptosis was more frequent in glomeruli of diabetic wild-type mice (FVwt, arrows) than in diabetic heterozygous (FVLr/q, DM, arrows) or homozygous (FVLq/q, DM, arrows) FVL mice. Exemplary images of TUNEL stain (A) and bar graph (B) summarizing results. Brown indicates TUNEL-positive cells detected by HRP-DAB reaction; blue, hematoxylin counterstain; n ≥ 7 for each group. (C-D) Expression of apoptosis regulators in renal cortex extracts. Bar graph (top) and representative immunoblot (bottom) showing p53 (C) and Bax (D) expression in renal cortex tissue samples summarizing results (n ≥ 7 for each group). Scale bar indicates 15 μm (A). C indicates nondiabetic, control mice (white bars); DM, diabetic mice (black bars); means ± SEM are shown; *P < .05; **P < .01 (ANOVA).