Cytokine release into culture supernatants during in vitro differentiation of FVIII-specific memory B cells into anti-FVIII ASC. CD138⁻ spleen cells were obtained from hemophilic mice treated with 4 weekly doses of 200 ng of FVIII and restimulated with stimulating (10 ng/mL) and inhibiting (1 and 20 μg/mL) concentrations of FVIII in the presence of medium (control), 100 ng/mL, or 1000 ng/mL CpG-ODN. Culture supernatants were taken after 6 days of culture and analyzed for cytokines. Arithmetic means of 2-5 experiments are presented. Medium control without FVIII (○); 10 ng/mL FVIII (●); 1 μg/mL FVIII (▴); and 20 μg/mL FVIII (◇).