Mouse VWF antigen levels after hydrodynamic injection. VWF knockout mice expressing wild-type, R1205H, Y1584C, or wild-type and mutant mVWF were sampled after thydrodynamic injection (N ≥ 10). VWF:Ag levels were determined via ELISA. (A) VWF:Ag levels from days 2 to 14 after hydrodynamic injection. (B) VWF:Ag levels from days 14 to 42 after hydrodynamic injection. Symbols represent means with SEM error bars. (C) VWF:Ag levels for days 21 and 28. Each circle represents data from a single mouse; bars represent the mean VWF:Ag value.