Thrombogenesis in response to ferric chloride injury. VWF knockout mice expressing VWF cDNA after hydrodynamic injection with VWF:Ag levels between 0.5 and 2.5 U/mL were evaluated using 10% ferric chloride injury to the cremaster. (A) Time to a stable occlusive thrombus. The experiment was stopped at 40 minutes, mice that failed to occlude were recorded as 40 minutes. (B) Platelet accumulation via mean relative fluorescence intensity. Mean with SEM was graphed for each dataset. (C) Total platelet accumulation. Area under the curve was determined. Each circle represents a single mouse; bars indicate mean.