Identification and characterization of NF-κB family members in DLBCL subsets. (A) Nuclear extracts purified from DLBCL cell lines (GCB-like and ABC-like, as indicated) were subjected to Western blot analysis with p50, p52, p65, c-rel, relB, and lamin B antibodies. (B) Cytoplasmic extracts purified from DLBCL cell lines (GCB-like and ABC-like, as indicated) were analyzed by Western blot analysis with phospho-IκBα and actin antibodies. (C) 6 DLBCL cell lines (4 GCB-like and 2 ABC-like) were used to purified cytoplasmic and nuclear extracts and were subjected to Western blot analysis with p52, relB, lamin B (nuclear marker), and actin (cytoplasmic marker) antibodies.