![Figure 6. The role of TRAF3 in the stabilization of NIK in DLBCL cells. (A) Cell lysates from a representative GCB-like (MS) or ABC-like (HB) DLBCL cells transfected with a TRAF3 expression vector or a control empty vector for 48 hours were probed with TRAF3 or NIK antibodies by Western blot analysis. Actin was used as a loading control. Relative protein level of each target molecule was measured with ImageJ densitometer software and normalized to the actin level. (B) Cell lysates from a representative GCB-like (MS) or ABC-like (HB) DLBCL cells transfected with TRAF3 shRNA or negative control shRNA were probed with TRAF3 or NIK antibodies by Western blot analysis. Actin was used as a loading control. Relative protein level of each target molecule was measured with ImageJ densitometer software and normalized to the actin level. (C) DLBCL cells (GCB-MS and SUDHL-4; ABC-HB and OCI-Ly3) were transfected with TRAF plasmid, TRAF3 shRNA, or negative control shRNA and cell proliferation was analyzed by [3H]-thymidine incorporation assays in vitro for 72 hours. The data shown are the means and ranges of triplicate samples relative to control samples of 2 independent experiments. Error bars represent SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/1/10.1182_blood-2010-06-290437/4/zh89991063130006.jpeg?Expires=1735872311&Signature=Ql8lHMr5hxAwJVjV2JsF4EPMM-dkhGxwr9LVo~uj6HxhfQKzhAsbVqoDl90wU6CBa~i6fj~3gotC~H8Vl2SPjMElR3Ow-M-s16jz1blrC0O3E2~WdGjK0VbEOpV63b7PlUPWPNis57zbkj-b2hVLMoWqfd7jbAqCAq8~pjmSzW7fX~VBQiPjlt1kxlmRwSJQeNpI9dQxL5pWiGtlddN7PAOrV0xTAJ-7FgKXdrLFOTthyEut8JUaRL3VGzNAMK2L8477vzWLYN8JgmeN6M42D7G~0GPylv1cqlGpWzWa1J3g2BjAX-hbXMQStBIoWpwJCXgjb5aJpquEiXPhl66oWQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The role of TRAF3 in the stabilization of NIK in DLBCL cells. (A) Cell lysates from a representative GCB-like (MS) or ABC-like (HB) DLBCL cells transfected with a TRAF3 expression vector or a control empty vector for 48 hours were probed with TRAF3 or NIK antibodies by Western blot analysis. Actin was used as a loading control. Relative protein level of each target molecule was measured with ImageJ densitometer software and normalized to the actin level. (B) Cell lysates from a representative GCB-like (MS) or ABC-like (HB) DLBCL cells transfected with TRAF3 shRNA or negative control shRNA were probed with TRAF3 or NIK antibodies by Western blot analysis. Actin was used as a loading control. Relative protein level of each target molecule was measured with ImageJ densitometer software and normalized to the actin level. (C) DLBCL cells (GCB-MS and SUDHL-4; ABC-HB and OCI-Ly3) were transfected with TRAF plasmid, TRAF3 shRNA, or negative control shRNA and cell proliferation was analyzed by [3H]-thymidine incorporation assays in vitro for 72 hours. The data shown are the means and ranges of triplicate samples relative to control samples of 2 independent experiments. Error bars represent SD.
