The effects of C5a and PI3Kdelta modulation on neutrophil transmigration. (A) Transmigration by neutrophils across a 3-μg transwell membrane toward an fMLF target following pretreatment with vehicle control, 180nM IC87114 or 30μM 740 Y-P. Negative control is without fMLF. P = .0029 by Kruskal-Wallis; *P < .05, **P < .01, NS P > .05 by Dunn posthoc test. (B) Transmigration by neutrophils across a 3-μg transwell membrane toward an fMLF target following pretreatment with vehicle control, 100nM C5a, 180nM IC87114 followed by 100nM C5a, or 100nM C5a followed by 0.3nM GM-CSF. Negative control is without fMLF. P = .0005 by Kruskal-Wallis; *P < .05, **P < .01, NS P > .05 by Dunn posthoc test.