EVI1 acute myeloid leukemia patients (EVI1 AMLs) have a unique genome-wide methylation profile compared with CD34⁺ normal bone marrow samples (CD34⁺ NBM). (A) Dendrogram representing a hierarchical clustering (i) and a principal component analysis (ii) in 8 CD34⁺ NBM blasts and 26 EVI1 AMLs. (B) Volcano plot showing the methylation difference comparing the 26 EVI1 AMLs to 8 CD34⁺ NBM samples with corresponding moderated t test P values. Probe sets that were significantly hypermethylated (P < .001 and methylation difference less than −1.5) are shown in red; probe sets that were significantly hypomethylated (P < .001 and methylation difference larger than 1.5) are shown in green. Significant probe sets that did not have an absolute methylation difference larger than 1.5 are depicted in blue. (C) Heatmap showing the methylation levels (Log (HpaII/MspI)) of differentially methylated genes (rows) in EVI1 AMLs and CD34⁺ NBM cases (columns). (D) Bar plots showing the percentages of genes containing (green) CG clusters and CpG islands and those not overlapping (gray) in all genes on the HELP array and in the EVI1 AML differentially methylated genes. A χ² test P value is shown per panel.