EVI1 interacts with DNMT3A and DNMT3B. (A) Western blot analysis using anti-FLAG antibodies shows the input of the immunoprecipitation of transfected 293T cells and the pulldown using anti-HA. (B) Confocal microscopy of 293T cells transfected with HA-tagged EVI1 (green) and FLAG-tagged DNMT3A and DNMT3B (red). (C) Western blot for EVI1 on lysates from SB1960CB cell line. The left lane shows the input band; the second and third lanes show EVI1 staining after immunoprecipitation with IgG control and anti-DNMT3A, respectively.