Figure 4
Figure 4. Effects of leukocyte serprocidins on the cleavage of CD11b and the detachment of adherent PMNs from immobilized FBG. (A) The detachment of adherent PMNs. The fMLP (10−7M)–induced PMN adhesion to immobilized FBG was performed in the presence of NE inhibitor (10μM), anti-NE mAb NP57 (50 μg/mL) or serine protease inhibitor ABESF (50μM). (B) The cleavage of CD11b by NE or other serprocidins. All PMNs (adherent or detached) were collected and blotted with antibody R7928A. Data represent 3 or more independent experiments. (C) “Initial” detachment of individual adherent PMNs measured using the Zigmond chamber assay (n = 8). (Inset) Phase contrast images of migrating PMNs in the presence (top) or absence (bottom) of AEBSF. Note that in the presence of AEBSF, PMN has a typical extended “tail” (arrowhead).

Effects of leukocyte serprocidins on the cleavage of CD11b and the detachment of adherent PMNs from immobilized FBG. (A) The detachment of adherent PMNs. The fMLP (10−7M)–induced PMN adhesion to immobilized FBG was performed in the presence of NE inhibitor (10μM), anti-NE mAb NP57 (50 μg/mL) or serine protease inhibitor ABESF (50μM). (B) The cleavage of CD11b by NE or other serprocidins. All PMNs (adherent or detached) were collected and blotted with antibody R7928A. Data represent 3 or more independent experiments. (C) “Initial” detachment of individual adherent PMNs measured using the Zigmond chamber assay (n = 8). (Inset) Phase contrast images of migrating PMNs in the presence (top) or absence (bottom) of AEBSF. Note that in the presence of AEBSF, PMN has a typical extended “tail” (arrowhead).

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