Effects of serprocidins on mouse PMN CD11b shedding and PMN detachment during chemotaxis. (A) Cleavage of CD11b in migrated PMNs collected from peritoneal lavages of mice with zymosan peritonitis. PMNs from mouse BM served as controls. (B) Effects of serine protease inhibitors on mouse PMN influx during zymosan peritonitis. PMNs were collected at 3 hours after zymosan injection. (C) Effects of serine proteases and protease inhibitors on mouse PMN transfilter migration. The transmigration of BM PMNs was induced by 5 × 10−6M fMLP. (Inset) Cleavage of PMN CD11b during PMN transfilter migration. Note that beige mouse PMNs had a significantly less CD11b cleavage. (D) Relative migration speed of mouse PMNs detected using the Zigmond chamber assay. PMN migration along immobilized FBG in the presence or absence of protease inhibitors (10μM each) or antibody (25 μg/mL) was measured within 30 minutes. (E) The impairment of PMN detachment in beige mice and the restoration of beige mouse PMN detachment by adding 1μM extracellular elastase (n = 8). Data represent the mean ± SD of 3 independent experiments, each performed in triplicate. *P < .05; **P < .01.
