Redox balance is disrupted in AML cells by GO-203 treatment. (A) MOLM-14 cells were left untreated, and treated with 5μM GO-203 or 5μM CP-2 each day for 3 days. The cells were then incubated with c-H₂DCFDA for 30 minutes. Fluorescence of oxidized DCF was measured by flow cytometry (left). The results are expressed as the relative H₂O₂ level (mean ± SD for 3 determinations) compared with that obtained with control cells (right). (B) MV4-11 cells were left untreated, and treated with 5μM GO-203 or 5μM CP-2 each day for 3 days. Oxidation of DCF was measured by flow cytometry. The results are expressed as the relative H₂O₂ level (mean ± SD for 3 determinations) compared with that obtained with control cells. (C-D) MOLM-14 (C) and MV4-11 (D) cells were left untreated, and treated each day with 5μM GO-203 or 5μM CP-2. The cells were harvested at the indicated times and analyzed for GSH levels. The results are expressed as GSH levels/10⁶ cells.