GO-203 induces late apoptosis/necrosis of AML cells. (A) MOLM-14 cells were left untreated, and treated with 5μM GO-203 or 5μM CP-2 each day for 3 days. The cells were stained with PI and annexin V, and analyzed by flow cytometry. The percentage of MOLM-14 cells staining with both PI and annexin V is indicated in the upper right panels. (B-D) MOLM-14 (B), MV4-11 (C) and AML patient 1 (D) cells were left untreated, and treated each day with 5μM GO-203 or 5μM CP-2. The MOLM-14, MV4-11 and AML patient 1 cells were harvested on days 3, 3, and 4, respectively. The results obtained from staining with PI/annexin V are expressed as the percentage of cells (mean ± SD of 3 determinations) with late apoptosis/necrosis (left). The cells were also seeded in agar in the absence of drug and colonies were counted at 14 days. The results are expressed as the number of colonies (mean ± SD of 3 determinations) (right).