Most CXCL12⁺ cells in metastatic melanoma tissues are CD209⁺ and CD163⁺ tumor-associated macrophages that display a perivascular location and express CXCR4. Metastatic melanoma tissues were stained with anti-CXCL12 and the following tissue markers: (A) melanocytic cells (HMb45) and myofibroblasts (CD90); (B) endothelial cells (VE-cadherin, VE) and myofibroblasts (CD90); (C) macrophages (CD209) and melanocytic cells (HMb45); and (D) quantification of CXCL12 expression in macrophages CD163⁺ was given as percentage. Several melanoma metastases were analyzed: #45 lung metastasis; #73, 79, 82, and 138 lymph node metastasis; and #98 skin metastasis. (E) Macrophages (CD163). (F) Macrophages (CD163), endothelial cells (Factor VIII), and TIE-2 positive cells. (G) Macrophages (CD163) and CXCR4⁺ cells (tumor and macrophages).