CXCL12 induces the proangiogenic factors VEGF and CCL1. (A) mRNA extracted from PBMos stimulated with CXCL12 (100 ng/mL) or unstimulated (control) was analyzed by microarrays. The ratios of the log2 intensity data of CXCL12-treated cells versus Control for some M1 (top histogram) and M2 (bottom histogram) genes are shown. Mean ± SD; n = 3. (B) ELISA quantification of secreted VEGF (black filled histogram) and IL-10 (gray histogram) from peripheral blood monocytes cultured for 48 hours. Cells were treated with AMD3100 and/or CCX733 as described in Figure 2D. Mean ± SE; n = 3; *P < .05. (C) Time course of CCL1 mRNA expression (qPCR) from PBMos stimulated with CXCL12 (100 ng/mL). Results were normalized to GAPDH levels. Fold regulation relative to control untreated cells is represented. Mean ± SD; n = 3. (D) Flow cytometric analysis of intracellular CCL1 protein expression in freshly isolated PBMos cultured for 12 hours (control, shaded profile) compared with CXCL12-stimulated PBMos (black line). Control isotype (discontinuous line). A representative experiment of 3 is shown.