American Society of Hematology

Figure 6

$Figure 6. Stabilin-1 and stabilin-2 in HSECs play important roles in blood decay of damaged RBCs in vivo. (A) FITC-labeled damaged RBCs were injected into clodronate liposomes (CL)–, stabilin shRNAs (shmStab1/2)–, or control shRNAs (shCont1/2)–treated mice. At the indicated times, 5 μL of venous blood was sampled from the tail vein and analyzed by flow cytometry for the fraction of fluorescent RBCs. Each experiment was independently performed 3 times. Data were normalized to the level at 1 hour after injection and represent mean ± SD for 6 mice per group. (B) FITC-labeled damaged RBCs were injected into stabilin shRNAs (shmStab1/2)– or control shRNAs (shCont1/2)–treated mice. At the indicated times, 5 μL of venous blood was sampled from the tail vein and analyzed by flow cytometry for the fraction of fluorescent RBCs. Each experiment was independently performed 3 times. Data were normalized to the level at 10 minutes after injection and represent mean ± SD for 6 mice per group.$

Stabilin-1 and stabilin-2 in HSECs play important roles in blood decay of damaged RBCs in vivo. (A) FITC-labeled damaged RBCs were injected into clodronate liposomes (CL)–, stabilin shRNAs (shmStab1/2)–, or control shRNAs (shCont1/2)–treated mice. At the indicated times, 5 μL of venous blood was sampled from the tail vein and analyzed by flow cytometry for the fraction of fluorescent RBCs. Each experiment was independently performed 3 times. Data were normalized to the level at 1 hour after injection and represent mean ± SD for 6 mice per group. (B) FITC-labeled damaged RBCs were injected into stabilin shRNAs (shmStab1/2)– or control shRNAs (shCont1/2)–treated mice. At the indicated times, 5 μL of venous blood was sampled from the tail vein and analyzed by flow cytometry for the fraction of fluorescent RBCs. Each experiment was independently performed 3 times. Data were normalized to the level at 10 minutes after injection and represent mean ± SD for 6 mice per group.

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