Lack of association of LG/HA-Y1 vector integration sites with proto-oncogenes after erythroid cell expansion. Integration sites from all 4 mice were pooled in this analysis. Integration sites isolated from Ter119⁺ (A) and CD45⁺ (B) BM cells are labeled according to the nearest RefSeq gene. The relative clone size was quantified by the number of times it was isolated with independent integration events catalyzed by MuA transposase in vitro. Proto-oncogenes, as annotated in the allOnco database.³⁴  are indicated by asterisks. The frequency of IS in which the nearest gene is an oncogene is not statistically different between erythroid and nonerythroid cells (P = .5523). (C) Integration site proximity to proto-oncogenes. The proportions of integration sites > 50 kb and < 50 kb from a proto-oncogene are shown. No significant differences in the number of integration sites found < 50 kb from a proto-oncogene were found between integration sites identified in this study and IS characterized in HCs transduced with the LG vector before and after transplantation (P > .05 with the 2-tailed Fisher exact test).