American Society of Hematology

Figure 3

$Figure 3. Characterization of D6active body cavity CD23+ B1 cells. (A) Left panels: Subfractionation of WT peritoneal cavity B1a (CD19+CD11b+CD5+), B1b (CD19+CD11b+CD5−) and B1c (CD19+CD11b−CD5+) cells by anti-CD23. Right panels: Overlaid CCL2AF647 uptake profiles of WT (black) and D6-deficient (KO, gray) peritoneal cavity CD23+ B1-cell subsets. (B) Surface immunophenotype of WT peritoneal cavity CD23+ (light gray) and CD23− (dark gray) B1-cell subsets. Data are shown as the average MFI for each marker ± SEM. (C) Forward scatter (FSC) and surface CD19 of WT peritoneal cavity CD23+ (23+) and CD23− (23−) B1 cell subsets. (D) Mean percentage ± SEM of each B1-cell subset expressing surface CD23 in WT peritoneal cavity and pleural cavity lavages (n = 5). ***P < .001. Flow cytometric profiles are representative of data from > 3 mice per genotype per experiment, with experiments repeated at least 3 times.$

Characterization of D6^active body cavity CD23⁺ B1 cells. (A) Left panels: Subfractionation of WT peritoneal cavity B1a (CD19⁺CD11b⁺CD5⁺), B1b (CD19⁺CD11b⁺CD5⁻) and B1c (CD19⁺CD11b⁻CD5⁺) cells by anti-CD23. Right panels: Overlaid CCL2^AF647 uptake profiles of WT (black) and D6-deficient (KO, gray) peritoneal cavity CD23⁺ B1-cell subsets. (B) Surface immunophenotype of WT peritoneal cavity CD23⁺ (light gray) and CD23⁻ (dark gray) B1-cell subsets. Data are shown as the average MFI for each marker ± SEM. (C) Forward scatter (FSC) and surface CD19 of WT peritoneal cavity CD23⁺ (23+) and CD23⁻ (23−) B1 cell subsets. (D) Mean percentage ± SEM of each B1-cell subset expressing surface CD23 in WT peritoneal cavity and pleural cavity lavages (n = 5). ***P < .001. Flow cytometric profiles are representative of data from > 3 mice per genotype per experiment, with experiments repeated at least 3 times.

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