Impact of D6 deficiency on B1-cell motility, B1-cell abundance, and serum anti-PC levels. (A) WT and D6-deficient (KO) peritoneal cavity B cells that migrated in response to 100nM CXCL13 plus 50nM CCL22 where indicated (+22) were enumerated by flow cytometry after staining with antibodies against CD19, CD11b, and CD5. Data are representative of at least 3 experiments. (B) Proportion of WT and KO peritoneal cavity CD5⁻CD11b⁻CD23⁺ B cells migrating toward 100nM CXCL13. A repeat experiment generated similar results. In panels A and B, data are presented as the mean number of live cells migrated (as percentage of input) ± SEM from at least 4 replicates. (C) Number of cells in the indicated B-cell subsets in peritoneal cavity lavage, omentum, and spleen of WT and D6-deficient (KO) mice as a percentage ± SEM of WT (WT average set to 100%; peritoneal cavity, 26 mice per genotype; omentum, 20 mice per genotype; spleen, 6 mice per genotype). 11b⁻5⁻ B cells were CD19⁺CD11b⁻CD5⁻; omental T cells were FSC^loSSC^loCD19⁻CD11b⁻CD23⁻CD5^hi. (D) Mean quantity of anti-PC Abs ± SEM in the serum of resting WT and KO mice (9 per genotype). Mean WT levels were set to 100%. Similar data were generated in a repeat experiment. *P < .05; **P < .01; ***P < .001. ns indicates not significant.