BMDCs do not differentiate into endothelial cells in dorsal excisional wounds. (A) The procedure for the dorsal excisional wound model combined with the GFP-bone marrow chimeric experiment. (B-H) Sectional immunohistochemistry for indicated antibodies. Grids in panels B through D indicate the fields in panels F through H, respectively. None of the CD31⁺ vascular endothelium is stained for GFP. (E,I) Whole-mount immunohistochemistry for indicated antibodies. A grid in panel E indicates the field in panel I. (J-K) Sectional immunohistochemistry for the tissues 7 days after wounding in Flk-1^+/EGFP mice (J) or LysM-Cre/Flox-CAT-EGFP mice (K). GFP was detected only in endothelial cells of Flk-1^+/EGFP mice and myeloid cells of LysM-Cre/Flox-CAT-EGFP mice. (L) FACS analysis for dissociated cells in wound tissues (day 7) of mice reconstituted with GFP⁺ bone marrow cells. None of GFP⁺ cells is positive for vascular endothelial-cadherin, and most GFP⁺ cells are positive for CD45. Bars represent 50 μm.