A major proportion of BMDCs recruited into wound tissues are macrophages in dorsal excisional wounds. (A-O) Sectional immunohistochemistry for indicated antibodies in unwounded and wounded tissues of mice reconstituted with GFP⁺ bone marrow cells. Most GFP⁺ cells located perivascularly (closed arrowheads) or in the vascular lumen (arrows) expressed F4/80. (P-Y) Sectional immunohistochemistry for the tissues 7 days after wounding in Flk-1^+/EGFP mice (P-T) or LysM-Cre/Flox-CAT-EGFP mice (U-Y). GFP was detected only in endothelial cells of Flk-1^+/EGFP mice (open arrowheads) and F4/80⁺ monocytes (arrows)/macrophages (closed arrowheads) of LysM-Cre/Flox-CAT-EGFP mice. (Z) FACS analysis for dissociated cells in wound tissues at day 7 indicated that approximately 70% of GFP⁺ cells are positive for CD11b, but F4/80 expression in FACS was weak and highly variable. Bar represents 50 μm.