LacZ staining of diaphragm and chest wall from Hprt-targeted mice carrying vWF2-lacZ or deletant VWF promoter-lacZ constructs. Diaphragms and chest walls were harvested from 6- to 8-week-old F2 male Hprt-targeted mice carrying vWF2-lacZ or deletant VWF transgenes and processed in parallel for whole-mount staining with X-Gal. In the left-most column (A,D,G,J,M), the diaphragm from the vWF2-lacZ transgenic mouse is on the right, and the diaphragm from the deletant VWF transgenic mouse is on the left. Panels in the second column (B,E,H,K,N) represent higher power images of the deletant VWF transgenic tissues shown A, D, G, J, and M, respectively. Wild-type vWF2-lacZ and all 5 deletant VWF transgenic mice demonstrate LacZ staining in macrovessels of skeletal muscle (diaphragm and intercostal muscle). However, note the loss of microvascular LacZ staining in vWF2-Del3-lacZ, vWF2-Del4-lacZ, and vWF2-Δ_843-620-lacZ tissues. Whole-mount tissues were analyzed under a Nikon SMZ-U dissecting microscope, and microphotographs were collected using a Nikon Coolpix 8400 camera.