LacZ staining of heart whole mounts and tissue sections from vWF2-lacZ and vWF2-Δ843-620-lacZ mice. Hearts were harvested from 6- to 8-week-old F2 male Hprt-targeted mice carrying vWF2-lacZ transgene (A-C) or vWF2-Δ843-620-lacZ transgene (identical to vWF2 except for an internal deletion of DNA sequences between −843 and −620; D-F) and processed in parallel for whole-mount (A,D) or cryosection (B-C,E-F) staining with X-Gal. vWF2-lacZ and vWF2-Δ843-620-lacZ transgenic hearts demonstrate LacZ staining in veins. However, note loss of capillary endothelial staining in the vWF2-Δ843-620-lacZ heart. Whole-mount hearts were analyzed under a Nikon SMZ-U dissecting microscope, and microphotographs were collected using a Nikon Coolpix 8400 camera. Tissue section images were obtained using a ×100 objective (B,E), ×60 objective (C), or ×40 objective (F). Lac-Z-stained sections were counterstained with eosin. Slides were analyzed under a Zeiss Axio Imager upright microscope, and photomicrographs were collected using a Zeiss Axiocam MRc camera and Axiovision 4.6.3 image acquisition software. Arrow, LacZ staining in venous endothelial cells. Asterisk, lumen of an artery.
