T-bet and GATA-3 induction in activated CD4⁺ T cells from Ras-deficient mice. (A) T-bet and GATA-3 expression in splenic CD4⁺ T cells left unstimulated (shaded curve) or stimulated with anti-CD3/CD28 antibodies for 48 hours (solid line). T-bet expression in cells stimulated in the presence of CpG-ODN is also shown (dotted line). Top and bottom values within histograms indicate the percentage and MFI, respectively, of cells in the marked region. (B) Unstimulated or anti–CD3-stimulated (48 hours) splenocytes were stained for expression of CD4 and IL-12Rβ2. (C) CXCR3 induction in splenic CD4⁺ T cells stimulated with anti-CD3 antibody for 72 hours. Numbers indicate percentage of cells in the gated region. Results in each panel are representative of ≥ 2 independent experiments.