Activated T lymphocytes can efficiently express either CD23.CAR or CD19.CAR. (A) CAR expression in CD19.CAR- and CD23.CAR-redirected T lymphocytes for one representative experiment of 10. Control NT T cells were also included. (B) Median expansion (-fold) of NT, CD19.CAR⁺, and CD23.CAR⁺ T lymphocytes generated from healthy donors and cultured for 9 days with IL-2 on retroviral-mediated gene transfer. Mean and SDs are shown for 8 different T-cell lines. (C) Phenotype of NT, CD19.CAR⁺, and CD23.CAR⁺ T lymphocytes generated from healthy donors and stained for 3 days on retroviral gene transfer. Data represent the means ± SD for 6 different T-cell lines.