CD23.CAR⁺ T lymphocytes generated from healthy donors and CLL patients produce inflammatory cytokines in response to CD23⁺ tumor cells. CD23.CAR⁺ T cells generated from healthy donors (A) and CLL patients (B) produced higher levels of TNF-β, TNF-α, and IFN-γ in response to allogeneic LCL cells compared with NT T cells. Data represent the means ± SD for 3 and 4 different T-cell lines generated from healthy donors and CLL patients, respectively. *P ≤ .05 and **P ≤ .005 comparing CAR⁺-transduced with NT T lymphocytes.