Vaccine-induced T cells treat large and established tumors. (A) Vaccination and treatment schema: C57BL/6 donors were vaccinated with CpG/H11. (B) Cohorts of C57BL/6 recipient mice (n = 5) received 25 × 10⁶ splenocytes from CpG/H11 vaccinated, CpG/EL4-vaccinated, or unvaccinated donors, and were followed for tumor growth. (C) Images of representative recipient mice on day 9 after tumor inoculation and on day 21. Results are representative of 2 independent experiments. (D) IFN-γ response assay to detect antitumor immune responses in recipient mice 15 days after transfer. Peripheral blood lymphocytes were cocultured with irradiated H11 tumor cells or the irrelevant tumor cell lines MC-38, LLC1, or B16.