Transplantation of GFP-labeled transgenic whole kidney marrow shows long-term engraftment in adult zebrafish. (A) FACS analysis of control Tg(β-actin:GFP) WKM cells showing the forward scatter vs side scatter profile from a representative donor animal. The erythroid gate is marked in red, the myeloid gate is green, the precursor gate is black, and the lymphoid gate is blue. (B) Histograms for GFP expression of cells within the myeloid, lymphoid, and precursor lineage gates for a representative Tg(β-actin:GFP) donor fish. The percentage of GFP⁺ cells in each lineage gate is shown. (C) Forward scatter (FSC) vs side scatter (SSC) profile of marrow from an animal 3 months after transplantation with 500 × 10³ marrow cells showing full reconstitution with donor cells. (D) Histograms for GFP expression of cells within the myeloid, lymphoid, and precursor gates for a representative transplant recipient fish analyzed 3 months after transplantation showing multilineage engraftment with GFP⁺ donor cells. (E) Kaplan-Meier survival curves of adult zebrafish transplanted with 500 × 10³ whole kidney marrow cells after graded doses of total body irradiation. (F) Percentage GFP⁺ cells in the myeloid and lymphoid populations of control Tg(β-actin:GFP) animals (left), in myeloid cells of transplant recipients (middle), and in lymphoid cells of transplant recipients (right). Each diamond represents an individual animal. Each host was transplanted with 500 × 10³ WKM cells after exposure to 20, 25, or 30 Gy of total body irradiation. Percentage of GFP⁺ cells in the myeloid gate (middle) and lymphoid gate (right) at 90 days after transplantation is shown. Percentages plotted correspond to raw data numbers. Red lines indicate the lower threshold for successful myeloid (> 4%) and lymphoid (> 0.6%) engraftment as determined by negative control animals. (G) Percentage GFP⁺ cells in the myeloid and lymphoid populations of WKM from secondary transplant recipients. Each unique symbol represents an individual animal.