Limiting dilution analysis reveals that survival and engraftment are tightly linked. Data from Table 1 were graphed for each cell dose in an HSC limiting dilution transplantation experiment (bars) and the SE calculated and shown. Recipients were injected with increasing numbers of marrow cells, and a constant number of peripheral blood carrier cells (105 per recipient). After 3 months, recipients were killed and the marrow was dissected for FACS analysis. GFP+ expression in the myeloid and lymphoid gates was used to determine donor engraftment. The percentage of animals surviving 90 days (A) and the percentage of animals engrafted (B) are shown. Transplanted marrow cell dose is depicted on the x-axis. The solid black line depicts the statistical fitted model.
