PCFT deficiency leads to impaired erythropoiesis and thrombopoiesis. (A) Representative May-Grünwald-Giemsa-stained cytocentrifuge preparations show accumulation of proerythroblasts and large basophilic erythroblasts (yellow arrows) in 4- to 6-week-old PCFT^−/− mice. Periodic acid Schiff-stained BM sections (original magnification 10×/0.3 NA dry objective) contain reduced number of megakaryocytes (green arrows) in PCFT^−/− mice than in WTs. (Inset) Megakaryocytes are less differentiated in the PCFT^−/− BM, as evidenced by marked reduction in cytoplasmic volume and nuclear lobulation. (B) Abnormal neutrophil precursors (giant metamyelocytes, yellow arrows) in the BM of PCFT^−/− mice (May-Grünwald-Giemsa staining of cytocentrifuge preparations). (C) Multilobed polymorphonuclear neutrophils in the PCFT^−/− blood smears. (D) Representative picture of submeningeal hemorrhage in a PCFT^−/− mouse (top panels) (original magnification 40×/0.75 NA dry objective). No evidence of deep intracerebral hemorrhage and calcification was found in PCFT^−/− brain sections by hematoxylin and eosin (bottom panel) and von Kossa staining (not shown). The hematoxylin and eosin low power image (original magnification 2×/0.08 NA dry objective) corresponds to the location of the insets at the right (original magnification 20×/0.75 NA dry objective; sagittal fissure above corpus callosum and ventrolateral cortex, respectively).