ARC19499 inhibition of TFPI in human plasma. (A) Representative thrombin generation curves with 1pM TF. Left, ARC19499 dose response in PNP. Right, ARC19499 dose response in TFPI-depleted plasma. (B-C) Effect of ARC19499 on thrombin generation with 1.0pM (♦) or 0.1pM (○) TF. The solid and dashed lines correspond to plasma in the absence of ARC19499 with 1.0 or 0.1pM TF, respectively. Left panel shows lag time; middle panel, ETP; right panel, peak thrombin. Thrombin generation activity was tested in PNP (B) and in TFPI-depleted plasma (C).