ChIP assay. Relative real-time PCR signal intensities of the ChIP assay, showing significant binding of TAL1, E47, GATA2, and RUNX1 near the 3-bp deletion polymorphism. represents transcription factor binding signals in the immediate vicinity of the 3-bp deletion polymorphism; binding signals at approximately 1.5 kb centromeric to the 3-bp deletion polymorphism; and binding signals at approximately 2.5 kb telomeric to the 3-bp deletion polymorphism. IgG indicates negative control using IgG for immunoprecipitation; H3, positive control using antibody against histone H3; and RT-PCR, using exon 3 primer set.