Preserved GVT activity of DNMAML-expressing T cells. (A) Lethally irradiated BALB/c mice (1000 rads) were transplanted with TCD BM and 5 × 10⁵ A20 B cell leukemia/lymphoma cells (H-2^d), with or without 2 × 10⁶ WT or DNMAML CD4⁺ T cells from B6 mice. Overall survival after transplantation. (B) In vivo detection of luciferase activity at day 20 after transplantation and injection of 5 × 10⁵ A20 luciferase cells (A20^TGL). (C) Lethally irradiated BALB/c mice (850 rads) were transplanted with TCD BM and 0.3, 1.0, or 3.0 × 10⁶ A20^TGL cells, with or without 2.0 × 10⁶ B6 WT or DNMAML CD4⁺ T cells. A representative example is shown for in vivo detection of luciferase activity at day 14, 28, and 46 after transplantation (5 mice/group). (D) Overall survival after transplantation for each dose of A20-TGL cells (n = 5 in each group). Recipients of TCD BM died of tumor progression. Recipients of WT CD4⁺ T cells controlled leukemia growth but died of severe GVHD. Recipients of DNMAML CD4⁺ T cells had markedly decreased GVHD, but were still able to control leukemia progression, resulting in improved overall survival.