DNMAML-expressing alloreactive CD4⁺ and CD8⁺ T cells show impaired activation of many but not all effector differentiation pathways. Quantitative reverse-transcription PCR was performed with RNA purified from alloreactive CFSE^low donor T cells at day 5 after transplantation of WT or Cd4-cre x ROSA26^DNMAMLf/+ (DNMAML) B6 splenocytes into unirradiated B6xDBA F1 (BDF1) recipients (Figure 6). Results are shown for alloreactive CD4⁺ and CD8⁺ T cells isolated from the spleen, after normalization with Hprt1. A relative value of 1 was assigned to WT CD4⁺ T cells. Error bars indicate the 95% confidence interval. (A) Expression of Notch target genes and effector cytokines; (B) expression of cytolytic molecules; and (C) expression of the master transcription factors of Th1, CD8⁺ effector T cell, and Treg differentiation. *P < .05.