Methylation patterns of pluripotent CB-HPCs. (A) Unsupervised hierarchical clustering of the 18 newly obtained methylation profiles together with 14 published profiles from other tissues. Defined cell types show a high overall similarity in the methylation patterns and thus cluster together. (B) Density plot of Illumina methylation scores of CB-HPCs. Although most markers are unmethylated, the plot also shows a pronounced peak of hypermethylated loci, consisting of 4950 markers with β values ≥ 0.8. (C) Ingenuity Pathway Analysis of markers with AVB ≥ 0.8 in CB-HPCs. The plot shows the 4 functional categories most significantly overrepresented in the hypermethylated loci in CB-HPCs. These categories are commonly associated with differentiated hematopoietic cells.