Analysis of age-dependent methylation patterns in HPCs. (A) Comparison of DNA methylation profiles of CB-HPCs and mPB-HPCs. Red dots indicate differentially methylated sites. (B) Validation of selected markers by 454 bisulfite sequencing. PCR amplification was performed on equimolar sample pools, and sequencing results are shown as in Figure 3D. Sequencing coverage ranged from 243 to 1657 reads, as indicated. Triangles indicate CpG dinucleotides that were interrogated on the array.