Loss of phenotypic HSPCs and myeloid progenitors in Shp2 knockout animals. (A) Representative FACS plots for lin⁻Kit⁺Sca1⁺ CD34⁻ (34⁻LSK) staining 2 weeks after final Poly-I:C injection. (B) Quantification of CD34⁻LSK cell frequency per 2 hindlimbs at indicated time points after final Poly-I:C injection (n = 3-4). (C) Quantification of CD34⁻LSK cell number per 2 hindlimbs at indicated time points after final Poly-I:C injection (n = 3-4). (D) LSK cell frequency in the spleen at indicated time points after final Poly-I:C injection (n = 3-4). (E) LSK cell frequency in the peripheral blood was not changed in mutant animal. Time points were after final Poly-I:C injection (n = 3-4). (F) CMPs, GMPs, MEPs, and CLPs frequency in the BM at 2 weeks after final Poly-I:C injection. CMPs were gated as Lin⁻Sca-1⁻Kit⁺FcgR^loCD34⁺. CLPs were gated as Lin⁻IL-7R⁻ Sca-1^loKit^lo. GMPs were gated as Lin⁻Sca-1⁻Kit⁺FcgR^hiCD34⁺. Megakaryocyte-erythrocyte progenitors were gated as Lin⁻Sca-1⁻Kit⁺FcgR^loCD34⁻. (G) Absolute numbers of CMPs, GMPs, MEPs, and CLPs at 2 weeks after final Poly-I:C injection (n = 4). ***P < .001, **P < .01,*P < .05, data are presented as mean ± SD.