Disturbed quiescence and increased apoptosis for LSK cells derived from Shp2^Δ/Δ BM. (A) Representative FACS plots illustrate BrdU/7-aminoactinomycin D (7-AAD) staining in LSK cells. (B) Quantification of S-phase or G₀/G₁-phase cell percentage in LSK subpopulation indicating more actively proliferating LSK cells in Shp2^Δ/Δ animals. Results were obtained from 3 independent experiments. (C) Quantification of G₀-G₁, S, and G₂-M phase cell percentage in 34⁻LSK subpopulation. (D) lin⁻ckit⁺Sca1⁻ and LSK cell but not lin⁻ population contained more Annexin V⁺ cells. (E, F) Elevated Annexin V⁺ 7-AAD⁻ and Annexin V⁺ 7-AAD⁺ percentage in Shp2^Δ/Δ 34⁻LSK cells (n = 4). ***P < .001, **P < .01,*P < .05, error bars are SD; see also supplemental Figure 3.