Silencing of miRNA-155 has no effect on HLA and costimulatory molecule expression in DCs. (A-B) Silencing of miRNA-155 in imDCs (A) and mDCs (B) did not affect HLA and costimulatory molecule expression. imDCs at day 4 were transfected with anti-miR-155, anti-miR-221 knockdown probes, or scramble probe controls. Two days later, cells were stained with the indicated antibodies (A). imDCs at day 6 were transfected with anti-miR-155, anti-miR-198 knockdown probes, or scramble probes followed by the maturation cocktail for 24 hours. Cells were stained with the indicated antibodies (B). x- and y-axes indicate fluorescein isothiocyanate (probe) and antibody intensity, respectively. (C-D) Deletion of miRNA-155 in DCs did not affect major histocompatibility complex and costimulatory molecule expression in murine imDCs and mDCs. CD11c⁺ cells were gated for analysis of expression of major histocompatibility complex class II or costimulatory molecules (C). BM-derived imDCs at day 6 were further treated with LPS (1 μg/mL) for 24 hours. Cells were then analyzed as in Figure 4C (D). Black dotted line indicates DCs from WT mice; solid gray line, DCs from miR-155^−/− (knockout [KO]) mice; and tinted line, isotope control. The data shown in every panel are representative of at least 3 independent experiments.