SAA activates distinct mitogenic pathways in T_reg. (A left) Real-time quantitation of ERK1/2 and AKT phosphorylation (pERK1/2 and pAKT) by phospho flow cytometry. T_eff (top) or T_reg (bottom) were labeled with CFSE. Data were collected at different time points (days 1, 4, and 7) during suppression assays with SJIA plasma (n = 6), HC plasma (n = 6) or complete media (FBS, n = 4). (Right) Representative FACS plots of pERK1/2 and pAKT in T_reg and T_eff in suppression assays. (B left) Expression of SOCS3 in T_reg and T_eff (n = 7). (Right) Representative FACS plots of expression of SOCS3 in T_reg and T_eff.