SOCS3 regulates mitogenic signaling cascade in T_reg in response to SAA. (A left) Percentages of proliferating T_reg and T_eff in suppression assays with HC plasma (n = 5) and SJIA plasma (n = 5). (Right) Percentages of proliferating forskolin-treated T_reg and untreated T_eff in suppression assays with HC plasma (n = 5) and SJIA plasma (n = 5). T_reg were rested in complete media or treated with forskolin for 24 hours before being used in these assays. (B left) Effects of T_reg specific-SOCS3 modulation on phosphorylation status of ERK1/2 and AKT by T_reg and T_eff in suppression assays. Data were collected at day 4 during suppression assays with SJIA plasma (n = 6), HC plasma (n = 6) or complete media (FBS, n = 4). (Right) Representative FACS plots of pERK1/2 and pAKT in T_reg and T_eff in suppression assays. Unpaired 2-tailed t tests (A,C,D) and paired 2-tailed t tests (B) were used for statistical analyses. Horizontal bars represented median values.