Deletion of Dot1l leads to the loss of HSCs and progenitors. (A) Staining profile of HSCs and progenitors 3 weeks after tamoxifen injection in Dot1l^F/F;CreER⁻ or Dot1l^wt/wt;CreER⁺ (Dot1l^+/+) and Dot1l^F/F;CreER⁺ (Dot1l^F/F) mice. HSC: lineage⁻, sca1⁺, ckit⁺, CD48⁻, CD150⁺. Common lymphoid progenitors: lineage⁻, IL-7Rα⁺, sca1^lo, ckit^lo, AA4.1⁺, CD135⁺. Megakaryocyte progenitors: lineage⁻, sca1^lo, ckit⁺, CD150⁺, CD41⁺. Granulocyte macrophage progenitors: lineage⁻, sca1^lo, ckit⁺, CD41⁻, CD16/32⁺, CD105⁻. Erythroid progenitors: lineage⁻, sca1^lo, ckit⁺, CD41⁻, CD16/32⁻, CD105⁺. Percentages are based on total live cell count. (B) Bar graph of population frequencies as defined in panel A. Dot1l deletion led to a statistically significant decrease in HSCs and progenitors in all lineages. Percentages are based on total live cell count. Data are mean ± SD.