Phenotypic analysis of HCV+MC+ patients' clonal B cells. PBMCs from one HCV−MC− (ECH 542, red) and one HCV+MC+ patient (LDU 125, blue) representative of 6 samples each are shown. (A) Staining of PBMCs with anti-CD20 and G6 mAbs. (B) Forward light scatter analysis of G6+ and G6− B cells from the HCV+MC+ person. Scanning electron micrographs of immunomagnetically sorted G6+ and G6− B cells from the HCV+MC+ person. (C-D) Scale bars on the low and high power magnifications represent 5 μm and 1 μm, respectively. Propidium iodide and Ki-67 staining of immunomagnetically sorted G6+ and G6− B cells from the HCV+MC+ person for Ki-67 and propidium iodide (E-F). Staining for cell surface CD10, CD21, CD27, CD11c, FCRL4, and IL-4R (G). Electron micrographs (original magnification ×10 000) were taken with a FEI Tecnai G2 Spirit BioTWIN Transmission Electron Microscope equipped with a Gatan ES500W Erlangshen CCD camera and DigitalMicrograph Software.
