KIR2DL2-expressing NK cells are inhibited by C1 as well as C2 ligands. Flow cytometric analysis of CD107 mobilization and intracellular IFN-γ on NK cells using 721.221 and HLA-transfected sublines Cw*0702 and Cw*0401 as target cell lines. (A) Histograms show CD107 expression of KIR2DL2/3⁺ cells from a donor with KIR2DL2 (genotype 12) and 1 donor without KIR2DL2 (genotype 1). (B) Functional inhibition was calculated by measuring reduction of CD107 mobilization NK cells in response to Cw*0401 (C2), Cw*0702 (C1)–transfected 721.221, and no targets (negative control). Values are calculated relative to NK cells cultured with untransfected 721.221. Analysis was gated on single-KIR2DL2/3⁺ NK cells in donors with (n = 6) or without (n = 5) KIR2DL2. Statistical analysis was performed by 2-tailed t test for *P < .05.