Adoptively transferred HIV-specific CD8⁺ T cells show phenotypic characteristics associated with CD8⁺ Tcm over time after transfer in vivo. (A) Expression of CD28 (x-axis) and CD62L (y-axis) on pentamer⁺ cells from patient 7707 before and after transfer. The phenotype of pentamer⁺ T cells (A*0301/QVPLRPMTYK-HIV-NEF) analyzed by flow cytometry 3, 14, 28, and 84 days after the second infusion are shown. Percentages of pentamer⁺CD8⁺ T cells that express CD28 and CD62L in each gated quadrant are shown in the top right aspect of each plot. (B top panel) Expression of CD28 (x-axis) and CD62L (y-axis) on pentamer⁺CD8⁺ T cells (B*0801/GEIYKRWII-HIV-GAG) from patient 7702 obtained 3, 7, 42, and 84 days after the second infusion. (B bottom panel) Pentamer⁺CD8⁺ T cells (HLA B*0702/IPRRIRQGL-HIV-ENV) from patient 7705 obtained 3, 14, 28, and 84 days after the second infusion.