LDN-193189 inhibits the induction of hepcidin by inflammation in zebrafish. (A) Human IL-6 protein was detected in homogenates of double transgenic (cmlc-IL-6) but not in those of WT zebrafish hearts (arrows). β-actin was used to control for protein loading. (B) Hepcidin mRNA levels were measured by qRT-PCR in the livers of 7-day-old WT and cmlc-IL-6 zebrafish treated with vehicle or LDN-193189 (6μM) for 16 hours. (n = 3, *,†P < .05 vs WT, #P < .05 vs cmlc-IL-6).