Reduced number of lymphoid cells in SHIP1^el20/el20 mice is non–cell-autonomous. (A) Donor contribution of myeloid (Mac-1⁺), B-cell (B220⁺), and T-cell (CD8⁺) lineages in peripheral blood of recipient mice 4 weeks after transplantation with a 1:1 ratio of donor (SHIP1^+/+ or SHIP1^el20/el20) and wild-type CD45.1 competitor BM cells. Bar graphs represent the mean ± SD calculated from 3 recipients of each donor genotype. (B) Donor contribution 12 weeks after transplantation. Mice were killed to determine donor contribution to the BM myeloid (Mac-1⁺), BM B-cell (B220⁺), and thymic T-cell (DP) lineages. Bar graphs represent the mean ± SD calculated from 3 recipients of each donor genotype. (C) Total numbers of DP thymocytes and pre-B cells in mice 12 weeks after transplantation with a 1:1 ratio of donor (SHIP1^+/+ or SHIP1^el20/el20) and wild-type CD45.1 competitor BM cells. The relative contribution of competitor cells is shown.