Increased IL-6 production and PI3K/mTOR signaling in SHIP1^el20/el20 mice. (A) IL-6 levels in serum from 4- to 5-week-old mice. Bar graph represents the mean ± SD of at least 3 mice for each genotype. *P < .05, 2-tailed Mann-Whitney test. **P < .01, 2-tailed Mann-Whitney test. (B) IL-6 levels in resting BMMΦs. Bar graph represents the mean ± SD of at least 6 samples for each genotype. *P < .05, 2-tailed Mann-Whitney test compared with SHIP1^+/+ control. ND indicates not detected. (C) IL-6 levels in resting BMMΦs after stimulation with LPS for the indicated times. Bar graph represents the mean ± SD of 6 independent samples for each genotype. ***P < .001, 2-tailed Mann-Whitney test compared with SHIP1^+/+ control. ND indicates not detected. (D) Western blot for pAkt Ser⁴⁷³ and total Akt in resting BMMΦs. (E) Western blot for pAkt Ser⁴⁷³ and total Akt in freshly isolated BAL cells from the same mice used for panel D. (F) Western blot for pS6 and total S6 in freshly isolated BAL cells from the same mice used for panel D except for the controls, which were from SHIP1^+/− heterozygous mice.