American Society of Hematology

Figure 3

$Figure 3. Flow cytometric analysis confirming multilineage engraftment. (A) Representative flow cytometry. The human graft is identified as the CD45+ fraction. The CD45+ human graft is composed of B-lymphoid cells (CD19+), myeloid cells (CD33+), and immature cells (CD34+). More than 50% of the CD19+ B-lymphoid population coexpress CD10 and CD34, indicative of a pre-B cell phenotype. (B) Multilineage and multiorgan human chimerism in the injected tibia, CL, spleen, and PB demonstrating migration of human cells from the injected tibia. No differences in the graft composition between the EV- and MLL-AF4–expressing CD34+ HSPCs were found (n = 35).$

Flow cytometric analysis confirming multilineage engraftment. (A) Representative flow cytometry. The human graft is identified as the CD45⁺ fraction. The CD45⁺ human graft is composed of B-lymphoid cells (CD19⁺), myeloid cells (CD33⁺), and immature cells (CD34⁺). More than 50% of the CD19⁺ B-lymphoid population coexpress CD10 and CD34, indicative of a pre-B cell phenotype. (B) Multilineage and multiorgan human chimerism in the injected tibia, CL, spleen, and PB demonstrating migration of human cells from the injected tibia. No differences in the graft composition between the EV- and MLL-AF4–expressing CD34⁺ HSPCs were found (n = 35).

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