Coagulation-induced GPVI shedding is metalloproteinase-dependent. sGPVI measured in recalcified citrated PRP in the absence (A-B) or presence of added TF (C-D) obtained from samples either resting or preincubated with a metalloproteinase inhibitor 100μM GM6001 (A,C), or with the ADAM10 inhibitor 2μM GI254023 (B,D). Conditions were as described in Figure 1 legend.